Week 8

I cover slipped my tissue this week:
     Steps for cover slipping:

•      Dehydrate the slides by putting them in 2 solutions of 70% ethanol, 2 solutions of 95%      ethanol, and 2 solutions of 100% ethanol for 5 minutes each
•      Put the slides in 3 solutions of xylene for 5 minutes each (xylene is in the fume hood)
•      Take a slide out of the last xylene with forceps and wipe the back
•      Smear a line of DPX along the slide and carefully slip the cover on the slide
•      Use the back of the forceps to push bubbles out 

Do one slide at a time.

I also completed a successful Nissl stain, and will work on analyzing that next week.