These are the pictures that will hopefully explain what I talked about last week.
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| This is how the tissue looks at the beginning. It's a little hard to see but the floating white things in each well are the tissue samples. Since I was mounting at the very beginning they were not separated in wells (they were just in one tray). Also, this tissue is human brain tissue which is bigger than the rat brain pieces, but I just wanted to show you how it looked before mounting. |
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| After I smoothed out each piece from the jumble (top picture), I had to put it in the order shown on this paper. |
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| This is an example of the final. Some of the pieces were ripped so it made it even more difficult than it already was to match it to the picture above because sometimes a cut off piece could look like it was from the top row, but it turned out that it was a piece of another part. |
Kinza,
ReplyDeleteAre these all still human sections, or rat? They look so tiny! Do they shrink during sectioning and processing? What is the next step in the process for you?
No these are mouse brain sections. Mouse brain is even smaller than the rat brain. This was the first time I did something on a section that was not human. The immuno unfortunately was unsuccessful so we couldn't do anything with it.
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